Journal article
Separation and characterization of proteins from green and etiolated shoots of rice (Oryza sativaL.): Towards a rice proteome
Abstract
Proteins extracted from green and etiolated shoots of rice were separated by two-dimensional polyacrylamide gel electrophoresis and relative molecular weights and isoelectric points were determined. The separated proteins were electroblotted onto a polyvinylidene difluoride membrane and 85 proteins were analyzed by a gas-phase protein sequencer. The N-terminal amino acid sequences of 21 out of 85 proteins were determined in this manner. …
Authors
Komatsu S; Muhammad A; Rakwal R
Journal
Electrophoresis, Vol. 20, No. 3, pp. 630–636
Publisher
Wiley
Publication Date
March 1, 1999
DOI
10.1002/(sici)1522-2683(19990301)20:3<630::aid-elps630>3.0.co;2-z
ISSN
0173-0835