Conference
Cardiac sarcoplasmic reticulum and sarcolemmal proteins separated by two‐dimensional electrophoresis: Surfactant effects on membrane solubilization
Abstract
We evaluated the use of the alkyaryl amidosulfobetaine zwitterionic detergent, designated as C8psi, to facilitate the solubilization of cardiac subcellular, membrane-associated proteins. Hearts from 7-week-old male Sprague-Dawley rats were isolated, and the left ventricles dissected and subsequently homogenized. The sarcolemma (SL) and the sarcoplasmic reticulum (SR) were isolated from different homogenate preparations originating from rat …
Authors
Macri J; McGee B; Thomas JN; Du P; Stevenson TI; Kilby GW; Rapundalo ST
Volume
21
Pagination
pp. 1685-1693
Publisher
Wiley
Publication Date
May 1, 2000
DOI
10.1002/(sici)1522-2683(20000501)21:9<1685::aid-elps1685>3.0.co;2-s
Conference proceedings
Electrophoresis
Issue
9
ISSN
0173-0835