Journal article
Selective cleavage of heparin using aqueous 2-hydroxypyridine: Production of an aldose-terminating fragment with high anticoagulant activity
Abstract
Unfractionated heparin (UFH) was partially depolymerized by heating at 115 degrees C with aqueous 2-hydroxypyridine. Compared to starting UFH, no significant loss of anticoagulant (anti-Xa) activity was observed. Products consisted of polysaccharide fragments and small quantities of ammonia, sulfate, and hexuronic acid. Fragments with aldose termini that reacted with [3H]NaBH4 (fragment A) were of relatively uniform size (6000 D) and increased …
Authors
Berry LR; Parmar N; Hatton MWC; Chan AKC
Journal
Biochemical and Biophysical Research Communications, Vol. 346, No. 3, pp. 946–957
Publisher
Elsevier
Publication Date
8 2006
DOI
10.1016/j.bbrc.2006.06.001
ISSN
0006-291X