Journal article
Multispectral multiplexed confocal FLIM for live cell imaging
Abstract
Spectrally resolved fluorescence lifetime imaging (FLIM) with high spatial precision offers comprehensive information on species localization and behavior. It is challenging to resolve weak fluorescence signals in multiple dimensions (spatial, spectral, and temporal) at high frame rates, especially in dynamic live cell processes, as photobleaching and phototoxicity limit acceptable photon count rates. We developed a multiplexed confocal FLIM …
Authors
Richards M; Osterlund EJ; Buendia AR; Malic N; Truant R; Fang Q
Journal
Journal of Physics Photonics, Vol. 7, No. 1,
Publisher
IOP Publishing
Publication Date
January 31, 2025
DOI
10.1088/2515-7647/ad936b
ISSN
2515-7647