Development of an electrochemical sensing technique for rapid genotyping of hepatitis B virus. Journal Articles uri icon

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abstract

  • OBJECTIVE: To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). METHODS: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by the binding of RuHex to phosphate groups of the DNA backbone before and after hybridization was used for distinguishing the different genotypes. RESULTS: During hybridization with genotype B; the charges detected at the No. 1 and 2 electrodes were significantly increased; while the charge at the No. 3 and 4 electrodes did not change significantly. During hybridization with genotype C; the charges detected at No. 3 and 4 electrodes were significantly increased; while the signals remained unchanged at the No. 1 and 2 electrodes. During hybridization with mixed genotypes (B and C); the charges detected at all four electrodes were significantly increased. The linear range of detection was 10(-7) to 10(-10) mol/L and the sensitivity for detecting mixed B (10%) or C (10%). CONCLUSIONS: Rapid genotyping of HBVs based on electrochemical sensing is simple, has good specificity; and can greatly reduce the cost. This method can be used for sensitive detection of mixed B and C HBV genotypes.

authors

  • Chen, Jinyuan
  • Weng, Shaohuang
  • Chen, Qingqiong
  • Liu, Ailin
  • Wang, Fengqing
  • Chen, Jing
  • Yi, Qiang
  • Liu, Qicai
  • Lin, Xinhua

publication date

  • March 20, 2014