Probing amino acid side chains of the integral membrane protein PagP by solution NMR: Side chain immobilization facilitates association of secondary structures

Solution NMR spectroscopy of large protein systems is hampered by rapid signal decay, so most multidimensional studies focus on long-lived ¹H-¹³C magnetization in methyl groups and/or backbone amide ¹H-¹⁵N magnetization in an otherwise perdeuterated environment. Herein we demonstrate that it is possible to biosynthetically incorporate additional ¹H-¹²C groups that possess long-lived magnetization using cost-effective partially deuterated or …