Chromatography of Drosophila tRNA on BD-cellolose Journal Articles

abstract

• Conditions for the amino acylation of Drosophila melanogaster tRNA with the 20 14C-labelled amino acids were established. Crude tRNA prepared from adult flies of wild-type D. melanogaster was chromatographed on BD-cellulose columns, and the fractions were assayed for amino acid acceptor activity. The elution profiles of the tRNAs are presented. The crude tRNA was chromatographed on a Sephadex G-100 column to isolate the 5 S RNA, which constituted 9% of this material. The 5 S RNA was further chromatographed on BD-cellulose and was resolved into one major and two minor peaks. The nucleotide composition of these peaks was determined by RNase T2 digestion and two-dimensional thin-layer chromatography.

authors

• White, Bradley
• Tener, GM

status

• published 

publication date

• June 1, 1973

has subject area

• 06 Biological Sciences (FoR)
• 08 Information and Computing Sciences (FoR)
• 11 Medical and Health Sciences (FoR)
• Amino Acids (MeSH)
• Amino Acyl-tRNA Synthetases (MeSH)
• Animals (MeSH)
• Biochemistry & Molecular Biology (Science Metrix)
• Carbon Isotopes (MeSH)
• Chromatography, DEAE-Cellulose (MeSH)
• Chromatography, Gel (MeSH)
• Chromatography, Ion Exchange (MeSH)
• Chromatography, Thin Layer (MeSH)
• Drosophila melanogaster (MeSH)
• Hydrogen-Ion Concentration (MeSH)
• RNA, Transfer (MeSH)
• Ribonucleotides (MeSH)
• Spectrophotometry, Ultraviolet (MeSH)
• Time Factors (MeSH)

published in

• Biochemistry and Cell Biology  Journal

keywords

• Amino Acids
• Amino Acyl-tRNA Synthetases
• Animals
• Carbon Isotopes
• Chromatography, DEAE-Cellulose
• Chromatography, Gel
• Chromatography, Ion Exchange
• Chromatography, Thin Layer
• Drosophila melanogaster
• Hydrogen-Ion Concentration
• RNA, Transfer
• Ribonucleotides
• Spectrophotometry, Ultraviolet
• Time Factors

Digital Object Identifier (DOI)

• 10.1139/o73-111

PubMed ID

• 4197825

start page

• 896

end page

• 902

volume

• 51

issue

• 6