FACS Analysis and Immunohistochemical Analysis of Human Myogenic Stem Cell Number and Cell‐cycle Kinetics in Response to Acute Myotrauma

In humans, myogenic stem cell (SC) enumeration is an important measurement used to determine the SC response in vivo to various physiological stimuli. The current standard for enumeration is immunohistochemistry (IHC) with antibodies against common SC markers (i.e. Pax7, NCAM). Fluorescent activated cell sorting (FACS) analysis may provide a more accurate determination of changes in the SC pool and provide additional analysis unachievable with …