Journal article
A fast, efficient, and scalable method for purifying recombinant SARS-CoV-2 spike protein
Abstract
Recombinant SARS-CoV-2 trimeric spike protein produced by mammalian cell culture is a potential candidate for a COVID-19 vaccine. However, this protein is much larger than most typical biopharmaceutical proteins and its large-scale manufacture is therefore challenging. Particularly, its purification using resin-based chromatography is difficult as the diffusive transport of this protein to and from its binding site within the pores of the …
Authors
Butani N; Xu Y; Pan S; Durocher Y; Ghosh R
Journal
Journal of Chromatography B, Vol. 1215, ,
Publisher
Elsevier
Publication Date
January 2023
DOI
10.1016/j.jchromb.2022.123579
ISSN
1570-0232