Journal article
The bldD Gene of Streptomyces coelicolor A3(2): a Regulatory Gene Involved in Morphogenesis and Antibiotic Production
Abstract
The bld mutants of Streptomyces coelicolor A3(2) are blocked at the earliest stage of sporulation, the formation of aerial hyphae, and are pleiotropically defective in antibiotic production. Using a phage library of wild-type S. coelicolor DNA, we isolated a recombinant phage which restored both sporulation and antibiotic production to strains carrying the single known bldD mutation. Nucleotide sequence analysis of a 1.3-kb complementing …
Authors
Elliot M; Damji F; Passantino R; Chater K; Leskiw B
Journal
Journal of Bacteriology, Vol. 180, No. 6, pp. 1549–1555
Publisher
American Society for Microbiology
Publication Date
March 15, 1998
DOI
10.1128/jb.180.6.1549-1555.1998
ISSN
0021-9193
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
Amino Acid SequenceAnti-Bacterial AgentsBacterial ProteinsBacteriophagesBase SequenceBlotting, NorthernChromosome MappingChromosomes, BacterialCloning, MolecularCodon, InitiatorCysteineDNA, BacterialDNA-Binding ProteinsGene ExpressionGene LibraryGenetic Complementation TestMolecular Sequence DataOpen Reading FramesPlasmidsPoint MutationPromoter Regions, GeneticProtein BiosynthesisRNA, BacterialRecombination, GeneticSequence AlignmentSequence Homology, Amino AcidSingle-Strand Specific DNA and RNA EndonucleasesSpores, BacterialStreptomycesTranscription FactorsTranscription, GeneticTyrosine