A Maxi Cl− Channel in Cultured Pavement Cells From the Gills of the Freshwater Rainbow Trout Oncorhynchus Mykiss

Primary cultures of pavement cells from the gills of a freshwater fish, the rainbow trout Oncorhynchus mykiss, have been studied for the first time using the patch-clamp technique. Gigaohm seals were obtained in approximately 95% of cells studied, and channel activity was evident in a high proportion (>90%). A large-conductance Cl- channel was evident in 6 % of cell-attached and in 31% of inside-out patches. Single-channel conductance in …