Experts has a new look! Let us know what you think of the updates.

Provide feedback
Home
Scholarly Works
Transformation with specific fragments of...
Journal article

Transformation with specific fragments of adenovirus DNAs I. Isolation of specific fragments with transforming activity of adenovirus 2 and 5 DNA

Abstract

DNA of human adenoviruses 2 and 5 was cleaved by the restriction endonucleases HsuI, BamHI, HpaI, and SmaI. The resulting fragments were separated and tested for their ability to transform primary baby rat kidney (BRK) cells, using the calcium technique. Fragments with transforming activity were obtained with endo's R·EcoRI (fragments A), BamHI (fragments B of Ad2 and A of Ad5 DNA), and HsuI (fragments G). The transforming fragments all …

Authors

Van der Eb AJ; Mulder C; Graham FL; Houweling A

Journal

Gene, Vol. 2, No. 3-4, pp. 115–132

Publisher

Elsevier

Publication Date

12 1977

DOI

10.1016/0378-1119(77)90012-9

ISSN

0378-1119