Tumorigenicity and viral gene expression in rat cells transformed by Ad 12 virions or by the EcoRI C fragment of Ad 12 DNA

Several rat kidney cell lines transformed either by Ad 12 virions or by the EcoRI C fragment (left 16%) of Ad 12 DNA have been characterized with respect to viral DNA content, viral gene expression, and tumorigenicity. Virion transformed cells contained viral DNA sequences from most regions of the viral genome while EcoRI C fragment transformed cells contained only part of the C fragment. All cell lines transcribed DNA sequences from the left 6.7% (HindIII F fragment) of the genome while some lines transcribed sequences extending into the adjacent HindIII H fragment. Virus transformed and DNA transformed lines induced tumors in immunocompetent rats with equal efficiency.