Foreign Gene Expression by Human Adenovirus Type 5-Based Vectors Studied Using Firefly Luciferase and Bacterial β-Galactosidase Genes as Reporters

Adenovirus (Ad) vectors have been used extensively to obtain high-level expression of foreign genes in mammalian cells and are currently being studied for use as live viral-vectored vaccines and as gene transfer vectors for gene therapy. Many Ad recombinants have been generated that express foreign genes inserted in early region 3 (E3); however, little has been done to study the importance for gene expression of regulatory sequences flanking …