Journal article
Development of a FLP/frt System for Generating Helper-Dependent Adenoviral Vectors
Abstract
Helper-dependent (HD) adenoviral vectors devoid of all viral coding sequences have a large cloning capacity and have been reported to provide long-term transgene expression in vivo with negligible toxicity, making them attractive vectors for gene therapy. Currently, the most efficient means of generating HD vectors involves co-infecting 293 cells expressing Cre with the HD vector and a helper virus bearing a packaging signal flanked by loxP …
Authors
Ng P; Beauchamp C; Evelegh C; Parks R; Graham FL
Journal
Molecular Therapy, Vol. 3, No. 5, pp. 809–815
Publisher
Elsevier
Publication Date
May 2001
DOI
10.1006/mthe.2001.0323
ISSN
1525-0016