Journal article
Development of a size-restricted pIX-deleted helper virus for amplification of helper-dependent adenovirus vectors
Abstract
Helper-dependent adenovirus vectors (hdAd), which are deleted of all viral protein-coding sequences, can mediate long-term expression of a therapeutic transgene and lead to life-long, phenotypic correction in animal models of genetic disease. Here, we describe a new system for the generation of hdAd, which utilizes the DNA size restrictions imposed on an Ad virion deleted of protein IX (pIX): such virions are reported to package up to only …
Authors
Sargent K; Ng P; Evelegh C; Graham F; Parks R
Journal
Gene Therapy, Vol. 11, No. 6, pp. 504–511
Publisher
Springer Nature
Publication Date
March 1, 2004
DOI
10.1038/sj.gt.3302107
ISSN
0969-7128