Further Codon Assignments in an Extremely Halophilic Bacterium using a Cell-Free Protein-Synthesizing System and a Ribosomal Binding Assay

Further codon assignments were made in a cell-free protein-synthesizing system from Halobacterium cutirubrum by use of the random copolyribonucleotides, poly CU, poly CI, poly CG, and poly AU. Polymers containing significant amounts of adenosine precipitated in the high salt concentration of the system and this prevented examination of many of the codons by this technique. An assay for codon recognition and interaction of aminoacyl-tRNA with …