Column liquid chromatographic determination of paroxetine in human serum using solid-phase extraction

A 0.5-ml aliquot of a serum sample, after the addition of a 100-microliters aliquot of a 5 micrograms/ml solution of dibucaine as the internal standard, is vortex-mixed with 0.5 ml of acetonitrile and centrifuged. The supernatant is applied to a 1-ml BondElut C18 silica extraction column conditioned with subsequent washings with 1 M HCl, methanol and water. After passing the sample at a slow rate, the column is washed twice with water and once …