A Novel Piezoelectric Biosensor for the Detection of Phytohormone β-Indole Acetic Acid

A novel piezoelectric immunosensor has been developed for the determination of β-indole acetic acid (IAA) in dilute solutions. The detection is based on competitive immunoreaction between a hapten (IAA) and an antigen (IAA-BSA, hapten-protein conjugation) bound to an anti-IAA antibody, immobilized on a quartz crystal microbalance (QCM). The frequency change (y) of the sensor caused by antigen is linearly related to the logarithm of the concentration of IAA (x) in the range of 0.5 ng/ml - 5 µg/ml with a regression equation of the form y = - 23x+ 151 (r = 0.9937).