Chemical variation from the neoantimycin depsipeptide assembly line Academic Article uri icon

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abstract

  • Here we report the biosynthetic pathway for the neoantimycin and present three novel neoantimycin analogues, neoantimycin D (1), E (2) and F (3), from this assembly system from Streptoverticillium orinoci. Identification of these novel neoantimycin variants was achieved by selective MS/MS interrogation of natural product extracts using diagnostic fragments of the known neoantimycins. Their structures, including the absolute configurations, were elucidated using a combination of NMR experiments, detailed MS/MS experiments and the advanced Marfey's method. The biosynthetic pathway of neoantimycin was dissected by genome sequencing data analysis for the first time, which includes a hybrid nonribosomal peptide synthetase (NRPS) and polyketide synthetase (PKS) assembly lines.

authors

  • Li, Xiang
  • Zvanych, Rostyslav
  • Vanner, Stephanie A
  • Wang, Wenliang
  • Magarvey, Nathan

publication date

  • September 2013