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Isolation of human iPS cells using EOS lentiviral...
Journal article

Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency

Abstract

Lentiviral vectors that express a fluorescent reporter and a selectable marker in pluripotent cells improve and simplify isolation of induced pluripotent stem cell lines in mouse and human.

Authors

Hotta A; Cheung AYL; Farra N; Vijayaragavan K; Séguin CA; Draper JS; Pasceri P; Maksakova IA; Mager DL; Rossant J

Journal

Nature Methods, Vol. 6, No. 5, pp. 370–376

Publisher

Springer Nature

Publication Date

January 1, 2009

DOI

10.1038/nmeth.1325

ISSN

1548-7091

Associated Experts

Mick Bhatia

Professor, Faculty of Health Sciences
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Labels

Fields of Research (FoR)

31 Biological sciences

Medical Subject Headings (MeSH)

AnimalsCell DedifferentiationCell DifferentiationCell SeparationDNA Transposable ElementsEmbryo, MammalianEmbryonic Stem CellsEnhancer Elements, GeneticFibroblastsGenes, ReporterGenetic VectorsGreen Fluorescent ProteinsHumansLentivirusMethyl-CpG-Binding Protein 2MiceMice, Inbred NODMice, Mutant StrainsMice, SCIDPluripotent Stem CellsPromoter Regions, GeneticRett SyndromeTeratoma
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