Synthesis and evaluation of photoaffinity probes directed at lanosterol 14α-demethylase (P-45014DM)

A series of aromatic azides which could act as potential photoaffinity probes for the yeast lanosterol 14α-demethylase (P-45014DM) were prepared. One of these, 1-[3H]-p-azidophenyl-2-(1-imidazolyl)ethanol, has been demonstrated to label purified P-45014DM from Saccharomyces cerevisiae. These results provide the framework for future labeling experiments.