Journal article
The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies
Abstract
A standardized data workflow is described for large-scale serum metabolomic studies using multisegment injection-capillary electrophoresis-mass spectrometry. Multiplexed separations increase throughput (<4 min/sample) for quantitative determination of 66 polar/ionic metabolites in serum filtrates consistently detected (coefficient of variance (CV) <30%) with high frequency (>75%) from a multi-ethnic cohort of pregnant women (n = 1,004). We outline a validated protocol implemented in four batches over a 7-month period that includes details on preventive maintenance, sample workup, data preprocessing and metabolite …
Authors
Shanmuganathan M; Kroezen Z; Gill B; Azab S; de Souza RJ; Teo KK; Atkinson S; Subbarao P; Desai D; Anand SS
Journal
Nature Protocols, Vol. 16, No. 4, pp. 1966–1994
Publisher
Springer Nature
Publication Date
April 2021
DOI
10.1038/s41596-020-00475-0
ISSN
1754-2189