The efficacy of electroporated plasmid vaccines correlates with long-term antigen production in vivo

We sought to determine whether in vivo electroporation might overcome the requirement for strong transcriptional promoters in plasmid vaccines. We tested plasmid vaccines containing viral promoters that exhibited a broad range of transcriptional activities by in vitro assay. In contrast to the in vitro observations, no difference in gene expression was measured in vivo 24h following injection when the plasmids were introduced via …