Decoding the metabolic landscape of pathophysiological stress-induced cell death in anucleate red blood cells.

BACKGROUND: In response to stress, anucleate red blood cells (RBCs) can undergo a process of atypical cell death characterised by intracellular Ca²⁺ accumulation and phosphatidylserine (PS) externalisation. Here we studied alterations in RBC metabolism, a critical contributor to their capacity to survive environmental challenges, during this process. MATERIALS AND METHODS: Metabolomics analyses of RBCs and supernatants, using …