Journal article
Detecting natural adaptation of the Streptococcus thermophilus CRISPR-Cas systems in research and classroom settings
Abstract
The authors describe here a core protocol with three variants, in which S. thermophilus cells are challenged with phages to study the adaptive nature of this bacterial CRISPR system. In addition, they elaborate on the use of this protocol in undergraduate education.
Authors
Hynes AP; Lemay M-L; Trudel L; Deveau H; Frenette M; Tremblay DM; Moineau S
Journal
Nature Protocols, Vol. 12, No. 3, pp. 547–565
Publisher
Springer Nature
Publication Date
March 2017
DOI
10.1038/nprot.2016.186
ISSN
1754-2189