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Isolation and characterization of cotiaractivase,...
Journal article

Isolation and characterization of cotiaractivase, a novel low molecular weight prothrombin activator from the venom of Bothrops cotiara

Abstract

In this study, we isolated a novel prothrombin activator from the venom of Bothrops cotiara, a Brazilian lance-headed pit viper (Cotiara, Jararaca preta, Biocotiara), which we have designated "cotiaractivase" (prefix: cotiar- from B. cotiara; suffix: -activase, from prothrombin activating activity). Cotiaractivase was purified using a phenyl-Superose hydrophobic interaction column followed by a Mono-Q anion exchange column. It is a single-chain …

Authors

Senis YA; Kim PY; Fuller GLJ; García Á; Prabhakar S; Wilkinson MC; Brittan H; Zitzmann N; Wait R; Warrell DA

Journal

Biochimica et Biophysica Acta, Vol. 1764, No. 5, pp. 863–871

Publisher

Elsevier

Publication Date

5 2006

DOI

10.1016/j.bbapap.2006.03.004

ISSN

0006-3002