Development of a mathematical model for evaluating the dynamics of normal and apoptotic Chinese hamster ovary cells Journal Articles uri icon

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  • AbstractA metabolic flux based methodology was developed for modeling the metabolism of a Chinese hamster ovary cell line. The elimination of insignificant fluxes resulted in a simplified metabolic network which was the basis for modeling the significant metabolites. Employing kinetic rate expressions for growing and non‐growing subpopulations, a logistic model was developed for cell growth and dynamic models were formulated to describe culture composition and monoclonal antibody (MAb) secretion. The model was validated for a range of nutrient concentrations. Good agreement was obtained between model predictions and experimental data. The ultimate goal of this study is to establish a comprehensive dynamic model which may be used for model‐based optimization of the cell culture for MAb production in both batch and fed‐batch systems. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011


  • Naderi, Saeideh
  • Meshram, Mukesh
  • Wei, Catherine
  • McConkey, Brendan
  • Ingalls, Brian
  • Budman, Hector
  • Scharer, Jeno

publication date

  • September 2011