A rapid biofabrication technique for self-assembled collagen-based multicellular and heterogeneous 3D tissue constructs

Although monolayer cell culture models are considered as gold standard for in vitro modeling of pathophysiological events, they cannot reconstruct in vivo like gradient of gases and nutrients and lack proper cell-cell and cell-matrix interactions. Spherical cellular aggregates, otherwise known as multicellular spheroids, are widely used as three-dimensional in vitro models to mimic natural in vivo cellular microenvironment for applications such …