Image analysis of the extracellular matrix Journal Articles

abstract

• AbstractThe epiphyseal growth plate and articular cartilage matrices were preserved by slam freezing and freeze substitution to optimally retain the native organization for both cellular and matrix components. These specimens were stained and examined using conventional electron microscopic methods. The highly integrated, proteoglycan‐rich matrices were examined by computer image analysis using such parameters as distribution, connectivity, orientation, and a variety of morphometric analyses. Also, different aspects of electron tomography and 3D rendering of matrix vesicles and their associated mineral deposits from epiphyseal growth plates and turkey leg tendons are presented. © 1994 Wiley‐Liss, Inc.

authors

• Arsenault, Larry
• Kohler, Derek M

status

• published 

publication date

• August 1994

has subject area

• 0299 Other Physical Sciences (FoR)
• 0601 Biochemistry and Cell Biology (FoR)
• 0912 Materials Engineering (FoR)
• Animals (MeSH)
• Cartilage, Articular (MeSH)
• Cattle (MeSH)
• Extracellular Matrix (MeSH)
• Freeze Substitution (MeSH)
• Freezing (MeSH)
• Growth Plate (MeSH)
• Image Processing, Computer-Assisted (MeSH)
• Mice (MeSH)
• Microscopy (Science Metrix)
• Proteoglycans (MeSH)
• Tendons (MeSH)
• Turkeys (MeSH)

published in

• Microscopy Research and Technique  Journal

keywords

• Animals
• Cartilage, Articular
• Cattle
• Extracellular Matrix
• Freeze Substitution
• Freezing
• Growth Plate
• Image Processing, Computer-Assisted
• Mice
• Proteoglycans
• Tendons
• Turkeys

Digital Object Identifier (DOI)

• 10.1002/jemt.1070280507

PubMed ID

• 7919528

start page

• 409

end page

• 421

volume

• 28

issue

• 5