Journal article
A study of phosphoglycerate kinase in human erythrocytes II. Kinetic properties
Abstract
Kinetic studies on phosphoglycerate kinase (EC 2.7.2.3) were performed in the forward reaction leading from 1,3-diphosphoglycerate to 3-phosphoglycerate. Substrate activation was observed at fixed levels of ADP or Mg2+ and varying concentrations of 1,3-diphosphoglycerate. A biphasic curve was obtained in both linear and double reciprocal plots demonstrating two Km values (Km1 1.9 - 10(-6) and Km2 9.8 - 10(6) M). Michaelis-Menten-type kinetics …
Authors
Ali M; Brownstone YS
Journal
Biochimica et Biophysica Acta, Vol. 445, No. 1, pp. 89–103
Publisher
Elsevier
Publication Date
August 1976
DOI
10.1016/0005-2744(76)90162-5
ISSN
0006-3002