Enzymatic Incorporation of 2-Amino-6-Vinylpurine Derivative Journal Articles uri icon

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  • Modified nucleotides are incorporated by enzymatic reactions into a DNA oligonulceotide to provide additional functionalities, which has shown potential applications in a wide variety of fields. A large number of modified nucleotide triphosphates have been incorporated to DNA or RNA by polymerases. We have previously described that the sulfide derivatives (1) of 2-amino-6-vinyl purine nucleoside (2, AVP) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we describe the preparation of the triphosphate derivative of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incorporation to DNA.


  • Imoto, S
  • Ali, Md M
  • Li, Yingfu
  • Sasaki, S
  • Nagatsugi, F

publication date

  • November 1, 2007