Journal article
Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors
Abstract
The prokaryotic tet operator (tetO) sequence was inserted at positions upstream and downstream of sequences encoding the Arabidopsis thaliana tRNAAUCLys or tRNAAUCTrp suppressor tRNAs, and tRNA expression in carrot protoplasts was measured by translational suppression of a nonsense codon in a luciferase reporter gene. Regulation of tRNA expression by the tetracycline repressor (tetR) occurred from genes with the tetO inserted at position −1 …
Authors
Ulmasov B; Capone J; Folk* W
Journal
Plant Molecular Biology, Vol. 35, No. 4, pp. 417–424
Publisher
Springer Nature
Publication Date
November 1997
DOI
10.1023/a:1005819007549
ISSN
0167-4412
Associated Experts
Fields of Research (FoR)
Medical Subject Headings (MeSH)
ArabidopsisBacterial ProteinsBase SequenceDaucus carotaEscherichia coli ProteinsGene Expression Regulation, PlantHumansLac RepressorsMolecular Sequence DataOperator Regions, GeneticProkaryotic CellsProtein BiosynthesisProtoplastsRNA, TransferRNA, Transfer, LysRNA, Transfer, SerRNA, Transfer, TrpRecombinant Fusion ProteinsRepressor ProteinsSuppression, GeneticTetracycline