Journal article
Enhanced protein expression by internal ribosomal entry site-driven mRNA translation as a novel approach for in vitro loading of dendritic cells with antigens
Abstract
Transfection of dendritic cells (DCs) with messenger RNAs (mRNAs) of tumor-associated antigens (TAAs) is a promising strategy for cancer vaccines. TAA mRNA can be generated by in vitro transcription using DNA encoding the TAA gene as a template. A cap analog is usually added upon in vitro transcription to stabilize mRNA and enhance the efficiency of mRNA translation. However, the inclusion of the cap analog correlates with significantly …
Authors
Tan X; Wan Y
Journal
Human Immunology, Vol. 69, No. 1, pp. 32–40
Publisher
Elsevier
Publication Date
January 2008
DOI
10.1016/j.humimm.2007.11.009
ISSN
0198-8859