Background and Objectives
Canadian Blood Services produces apheresis and buffy coat pooled platelet concentrates (
PCs) stored in bags produced by two different manufacturers (A and B, respectively), both made of polyvinyl chloride‐butyryl trihexyl citrate. This study was aimed at comparing Staphylococcus epidermidisadhesion to the inner surface of both bag types in the presence or absence of plasma factors. Materials and Methods
N= 2–6) of bags type A and B were left non‐coated (control) or preconditioned with platelet‐rich, platelet‐poor or defibrinated plasma ( PRP, PPPand Defib PPP, respectively). Each bag was inoculated with a 200‐ml S. epidermidisculture adjusted to 0·5 colony‐forming units/ml. Bags were incubated under platelet storage conditions for 7 days. After culture removal, bacteria attached to the plastic surface were either dislodged by sonication for bacterial quantification or examined in situby scanning electron microscopy ( SEM). Results
Higher bacterial adhesion was observed to preconditioned
PCbags than control containers for both bag types ( P< 0·0001). Bacterial attachment to preconditioned bags was confirmed by SEM. Bacteria adhered equally to both types of containers in the presence of PRP, PPPand Defib PPPresidues ( P> 0·05). By contrast, a significant increase in bacterial adherence was observed to type A bags compared with type B bags in the absence of plasma ( P< 0·05) [Correction added on 16 June 2017, after first online publication: this sentence has been corrected]. Conclusion
The ability of
S. epidermidisto adhere to preconditioned platelet collection bags depends on the presence of plasma factors. Future efforts should be focused on reducing plasma proteins' attachment to platelet storage containers to decrease subsequent bacterial adhesion.