Journal article
Transplantation of Articular Cartilage Following a Step-Cooling Cryopreservation Protocol
Abstract
Using a step-cooling cryopreservation protocol that held the tissue 60 min at -4 degrees C, 30 min at -8 degrees C, and 10 min at -40 degrees C before plunging into liquid nitrogen, we were able to get a substantial improvement in the magnitude and pattern of chondrocyte recovery following cryopreservation, achieving postthaw recoveries of 62 +/- 13%. These results are consistent with the hypothesis that ice growth within articular cartilage is …
Authors
Muldrew K; Novak K; Studholme C; Wohl G; Zernicke R; Schachar NS; McGann LE
Journal
Cryobiology, Vol. 43, No. 3, pp. 260–267
Publisher
Elsevier
Publication Date
November 2001
DOI
10.1006/cryo.2001.2349
ISSN
0011-2240