Conference
Exocytosis Studies in a Chromaffin Cell‐Free System
Abstract
We have developed a system for the real-time study of regulated exocytosis in living, cultured bovine adrenal chromaffin cells (BCCs). Exocytosis was monitored by the use of total internal reflection fluorescence (TIRF) microscopy to image single large dense-core secretory vesicles (LDCVs). Fluorescent labeling of LDCVs was achieved either with the membrane-permeant weak base, acridine orange (AO), or by transduction of BCCs so as to express a …
Authors
WIEGAND UK; DON‐WAUCHOPE A; MATSKEVICH I; DUNCAN RR; GREAVES J; SHIPSTON MJ; APPS DK; CHOW RH
Volume
971
Pagination
pp. 257-261
Publisher
Wiley
Publication Date
10 2002
DOI
10.1111/j.1749-6632.2002.tb04472.x
Conference proceedings
Annals of the New York Academy of Sciences
Issue
1
ISSN
0077-8923