Journal article
Optimized Dialysis and Protease Inhibition of Sputum Dithiothreitol Supernatants
Abstract
RATIONALE: Dithiothreitol (DTT) is commonly used to liquefy induced sputum samples before assessment of cytology, but causes reduction of disulfide bonds and denaturation of proteins.
OBJECTIVES: To process sputum supernatants containing DTT to enable quantification of cytokines and chemokines.
Authors
Erin EM; Jenkins GR; Kon OM; Zacharasiewicz AS; Nicholson GC; Neighbour H; Tennant RC; Tan AJ; Leaker BR; Bush A
Journal
American Journal of Respiratory and Critical Care Medicine, Vol. 177, No. 2, pp. 132–141
Publisher
American Thoracic Society
Publication Date
January 15, 2008
DOI
10.1164/rccm.200603-311oc
ISSN
1073-449X