Journal article
Improved DNA extraction from ancient bones using silica‐based spin columns
Abstract
We describe a simple method for extracting polymerase chain reaction-amplifiable DNA from ancient bones without the use of organic solvents. Bone powders are digested with proteinase K, and the DNA is purified directly using silica-based spin columns (QIAquick3, QIAGEN). The efficiency of this protocol is demonstrated using human bone samples ranging in age from 15 to 5,000 years old.
Authors
Yang DY; Eng B; Waye JS; Dudar JC; Saunders SR
Journal
American Journal of Biological Anthropology, Vol. 105, No. 4, pp. 539–543
Publisher
Wiley
Publication Date
April 1998
DOI
10.1002/(sici)1096-8644(199804)105:4<539::aid-ajpa10>3.0.co;2-1
ISSN
0002-9483