Skin-type Antifreeze Protein from the Shorthorn Sculpin,Myoxocephalus scorpius EXPRESSION AND CHARACTERIZATION OF A M r9,700 RECOMBINANT PROTEIN*

A cDNA clone encoding a presumptive antifreeze protein was isolated from a skin library from shorthorn sculpin, Myoxocephalus scorpius. The clone encodes a 92-residue mature polypeptide (sssAFP-2) without any signal and prosequence, which suggests an intracellular localization. It is the largest alanine-rich, alpha-helical type I antifreeze protein known. A recombinant fusion protein containing an N-terminal-linked His-tag was produced and …