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Integrated fragmentation of human IgG and...
Journal article

Integrated fragmentation of human IgG and purification of Fab using a reactant adsorptive membrane bioreactor separator system

Abstract

This article discusses an integrated separation-reaction-separation scheme for producing Fab fragment directly from human immunoglobulin G (hIgG) present in serum feed. The novel reactant adsorptive membrane bioreactor separator (or RAMBS) system used in the current study consisted of a stack of microporous adsorptive membranes held within a temperature controlled module. The membrane stack, in the presence of salt, selectively and reversibly …

Authors

Yu D; Ghosh R

Journal

Biotechnology and Bioengineering, Vol. 104, No. 1, pp. 152–161

Publisher

Wiley

Publication Date

9 2009

DOI

10.1002/bit.22362

ISSN

0006-3592