Biosynthetic utilization of photoreactive fatty acids by rat liver microsomes

The photoreactive omega-diazirinophenoxy derivatives of nonanoate, undecanoate, tridecanoate, and pentadecanoate were shown to be activated by rat liver microsomes to the corresponding acyl-CoA derivatives. The Km and Vmax for these fatty acid analogues were determined: the values obtained indicate that the addition of a photoreactive group to an alkyl chain has an effect similar to that of elongation of the chain by about seven carbons. …