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The effect of intracellular pH on long-chain fatty...
Journal article

The effect of intracellular pH on long-chain fatty acid uptake in 3T3-L1 adipocytes: evidence that uptake involves the passive diffusion of protonated long-chain fatty acids across the plasma membrane

Abstract

To understand the mechanism of long-chain fatty acid permeation of the plasma membrane in mammalian cells, the effects of changes in the cytoplasmic pH on the internalization of physiologically relevant, submicromolar concentrations of uncomplexed long-chain fatty acids were investigated in 3T3-L1 adipocytes. The acidification of the cytoplasm upon NH4Cl prepulsing of intact cells was accompanied by a rapid reduction of cellular long-chain …

Authors

TRIGATTI BL; GERBER GE

Journal

Biochemical Journal, Vol. 313, No. 2, pp. 487–494

Publisher

Portland Press

Publication Date

January 15, 1996

DOI

10.1042/bj3130487

ISSN

0264-6021