Journal article
Direct detection of vanA and vanB genes in clinical specimens for rapid identification of vancomycin resistant enterococci (VRE) using multiplex PCR
Abstract
Surveillance for vancomycin resistant enterococci (VRE) by culture can be labour intensive and time consuming. We have developed a multiplex polymerase chain reaction (MPCR) which can be performed directly on the clinical specimen. The assay allows sensitive detection of enterococci with vanA - and vanB -mediated resistance to vancomycin. DNA was purified from stool and rectal specimens using the XTRAX(TM)DNA Extraction Kit (Gull Labs). …
Authors
Petrich A; Luinstra K; Groves D; Chernesky M; Mahony J
Journal
Molecular and Cellular Probes, Vol. 13, No. 4, pp. 275–281
Publisher
Elsevier
Publication Date
August 1999
DOI
10.1006/mcpr.1999.0250
ISSN
0890-8508