Journal article
Vector-Derived Expression of Recombinant Rat Interleukin-6
Abstract
Rat interleukin-6 (IL-6) cDNA, coding for an important inflammation- and immune-regulatory polypeptide cytokine, was cloned into the novel expression vector pH6EX3 which directs the synthesis of inserted genes as a fusion protein with histidine hexapeptide (HH). The resultant vector (pRIL6.992) was shown to produce significant amounts of recombinant rat IL-6 fusion protein with HH at its N-terminus in various strains of Escherichia coli. The …
Authors
Braciak TA; Northemann W; Chong DK; Schroeder J-A; Gauldie J
Journal
Protein Expression and Purification, Vol. 7, No. 3, pp. 269–274
Publisher
Elsevier
Publication Date
5 1996
DOI
10.1006/prep.1996.0038
ISSN
1046-5928