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Nerve trophic effects: Partial purification from...
Journal article

Nerve trophic effects: Partial purification from chick embryo brains of proteins that stimulate protein synthesis in cultured newt blastemata

Abstract

Eighteen-day embryonic chick brain homogenates stimulate the incorporation of 14C-amino acids into proteins of cultured early to medium bud stage newt blastemata. DEAE-cellulose column chromatography of the homogenate yields a fraction which is highly active but unstable. Electrophoresis of the homogenates on “native” polyacrylamide gels (under nondenaturing conditions) yields two regions of activity on the gels when gel fractions are assayed …

Authors

Choo AF; Logan DM; Rathbone MP

Journal

Experimental Neurology, Vol. 73, No. 2, pp. 558–570

Publisher

Elsevier

Publication Date

8 1981

DOI

10.1016/0014-4886(81)90289-2

ISSN

0014-4886