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α-Actinin Accumulation in Epithelial Cells...
Journal article

α-Actinin Accumulation in Epithelial Cells Infected with Attaching and Effacing Gastrointestinal Pathogens

Abstract

Fluorescent F-actin staining utilizing phalloidin, a highly toxic mushroom poison, is used as an indirect test to detect attaching and effacing (AE) bacteria. A study was done to determine if accumulation of alpha-actinin in infected tissue culture cells is a consistent feature and whether it corresponds with the AE response. Rearrangement of alpha-actinin was detected using immunofluorescence microscopy by incubation of infected cells with a …

Authors

Ismaili A; Philpott DJ; Dytoc MT; Soni R; Ratnam S; Sherman PM

Journal

The Journal of Infectious Diseases, Vol. 172, No. 5, pp. 1393–1396

Publisher

Oxford University Press (OUP)

Publication Date

November 1, 1995

DOI

10.1093/infdis/172.5.1393

ISSN

0022-1899