Journal article
Ultrasensitive ATP Detection Using Firefly Luciferase Entrapped in Sugar-Modified Sol−Gel-Derived Silica
Abstract
Firefly luciferase (FL) was entrapped in sol-gel-derived silica containing precursors based on covalent linkage of d-gluconolactone or d-maltonolactone to (aminopropyl)triethoxysilane to form N-(3-triethoxysilylpropyl)gluconamide or N-(3-triethoxysilylpropyl)maltonamide. The enzyme was active and stable in this material and showed catalytic constants close to those in solution. As little as 20 amol ATP could be detected with the entrapped FL, …
Authors
Cruz-Aguado JA; Chen Y; Zhang Z; Elowe NH; Brook MA; Brennan JD
Journal
Journal of the American Chemical Society, Vol. 126, No. 22, pp. 6878–6879
Publisher
American Chemical Society (ACS)
Publication Date
June 1, 2004
DOI
10.1021/ja0490424
ISSN
0002-7863