Journal article
Passively Operated Microfluidic Device for Stimulation and Secretion Sampling of Single Pancreatic Islets
Abstract
A passively operated polydimethylsiloxane (PDMS) microfluidic device was designed for sampling of hormone secretions from eight individual murine pancreatic islets in parallel. Flow control was achieved using a single hand-held syringe and by exploiting inherent fluidic resistances of the microchannels (R(sampling) = 700 ± 20 kPa s mm(-3) at 37 °C). Basal (3 mM) or stimulatory (11 mM) glucose levels were applied to islets, with stimulation …
Authors
Godwin LA; Pilkerton ME; Deal KS; Wanders D; Judd RL; Easley CJ
Journal
Analytical Chemistry, Vol. 83, No. 18, pp. 7166–7172
Publisher
American Chemical Society (ACS)
Publication Date
September 15, 2011
DOI
10.1021/ac201598b
ISSN
0003-2700