Journal article
Identification of a novel Wee1 isoform
Abstract
We have identified a novel isoform of Wee1 kinase (Wee1i), which uses Met215 of Wee1 as its initiation codon. RT-PCR, Western blot, and in situ hybridization verified wee1i expression in mammalian cells, rat brain, and rat thymus. Recombinant and partially purified Wee1i from rat thymus displayed kinase activity comparable to or higher than Wee1. The N-terminal 214 residues of Wee1 facilitate its ubiquitin-dependent degradation to trigger …
Authors
Yu Y; Chen B; Chen Z; Fan C; Han Y; Zhang J; He L; Ingram A; Kapoor A; Wang JH
Journal
Biochimica et Biophysica Acta, Vol. 1729, No. 1, pp. 1–9
Publisher
Elsevier
Publication Date
May 2005
DOI
10.1016/j.bbaexp.2005.02.006
ISSN
0006-3002